【分享】免疫组化图片 图像分析 新方法 分享 （从文献中看到）
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各位站友：
我在一篇文献：“Adeno-associated virus-mediated anti-DR5 chimeric antibody
expression suppresses human tumor growth in nude mice”
2011年，中国医科院，北京。
看到一张免疫组化的分析结果，很新颖，和各位一起分享。如下：
Fig. 5. Apoptotic cell death in rAAV-Adximab infected tumor tissue.
(A) Paraffin-embedded sections of SMMC7721 and HCT116 tumor tissues were prepared from the mice at the
end of the experiment. Apoptotic cells in the tumor tissues were visualized by TUNEL assay. Brown colored cells
represent apoptotic cells. rAAV-EGFP was used as control (magnification, 400x).
(B) Apoptotic cells in the defining regions of interest (ROI) were quantified by using automated cell acquisition and the software for immunohistochemistry (Histoquest).
(C) TUNEL mean intensities in tumor tissue from the representative animal infected with rAAV-Adximab and
rAAV-EGFP, respectively. **p < 0.01.
我在一篇文献：“Adeno-associated virus-mediated anti-DR5 chimeric antibody
expression suppresses human tumor growth in nude mice”
2011年，中国医科院，北京。
看到一张免疫组化的分析结果，很新颖，和各位一起分享。如下：
Fig. 5. Apoptotic cell death in rAAV-Adximab infected tumor tissue.
(A) Paraffin-embedded sections of SMMC7721 and HCT116 tumor tissues were prepared from the mice at the
end of the experiment. Apoptotic cells in the tumor tissues were visualized by TUNEL assay. Brown colored cells
represent apoptotic cells. rAAV-EGFP was used as control (magnification, 400x).
(B) Apoptotic cells in the defining regions of interest (ROI) were quantified by using automated cell acquisition and the software for immunohistochemistry (Histoquest).
(C) TUNEL mean intensities in tumor tissue from the representative animal infected with rAAV-Adximab and
rAAV-EGFP, respectively. **p < 0.01.
