膀胱出口梗阻对肌浆网蛋白和基因表达的影响

文题：THE DECOMPENSATED DETRUSOR III: IMPACT OF BLADDER OUTLET OBSTRUCTION ON SARCOPLASMIC ENDOPLASMIC RETICULUM PROTEIN AND GENE EXPRESSION

作者：RAIMUND STEIN, CHAOLIANG GONG, JOEL C. HUTCHESON, DOUGLAS. CANNING AND STEPHEN A. ZDERIC
From the Division of Urology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania

杂志全名：THE JOURNAL OF UROLOGY
年份，卷（期）: 起止页码：2000 Sep;164(3 Pt 2):1026-30.
PMID: 10958733

英文摘要：Purpose: Regulation of calcium ion homeostasis has a significant role in smooth muscle
contractility. :)The sarcoplasmic endoplasmic reticulum, calcium, magnesium, adenosine triphosphatase
(SERCA) is a regulatory ion pump that may have a|) role in the functional outcome after
outlet obstruction. We investigate what correlation if any existed between SERCA protein and
gene expression, and the contractile properties in the same bladder.
Materials and Methods: Standardized partial bladder outlet obstructions were created in adult
New Zealand white rabbits, which were divided into control, sham operated and obstructed
groups. Muscle strip studies subcategorized the obstructed group into compensated (force greater
than 50% of control) and decompensated (force less than 50% of control). Microsomal membrane
and total RNA fractions were prepared from the same bladder tissue. Membrane proteins were
used for Western blot analysis :8)using a SERCA specific monoclonal antibody, and total RNA was
assessed with Northern blot analysis.
Results: The relative intensities of signals for the Western and Northern blots demonstrated a
strong correlation between protein and geneB) expression. Furthermore there was a strong association
between the loss of SERCA messenger RNA and protein expression and loss of bladder
function.
Conclusions: Bladder contractility after outlet obstruction is influenced in part by smooth
muscle cell ability to maintain calcium homeostasis via SERCA.:^) The loss of SERCA protein
expression is mediated by down-regulation in gene expression in the same bladder. These data
suggest that smooth muscle ion pump gene expression is in part mechanically (pressure work)
regulated.
KEY WORDS: bladder, rabbits, ca(21)-transporting atpase文题：

中文译文：目的：在平滑肌收缩中钙离子稳态调节有着重要作用。肌浆网上调节钙镁离子泵ATP酶（SERCA）可能在膀胱出口梗阻后功能改变中起作用。我们研究同一膀胱中SERCA的蛋白和基因表达与收缩特征之间是否存在相关性。

材料和方法：用成年新西兰白兔制作标准的部分膀胱出口梗阻，并分为对照、假手术组和梗阻组。通过肌条实验将梗阻组再分成代偿组（收缩强度大于对照50%）和失代偿组（收缩强度小于50%）。微粒体膜和总RNA 部分来自同一膀胱组织，膜蛋白用SERCA特异的单克隆抗体作Western blot分析，总RNA用Northern blot分析来评估。:})

结果：Western 和Northern印迹的相对信号强度证实在蛋白和基因表达间存在强相关性，而且在SERCA的RNA信息及蛋白表达丢失8D和膀胱功能的损失也存在强相关性。

结论：膀胱出口梗阻后的收缩特性部分的受平滑肌细胞中能维持钙稳态的SERCA影响。在同一膀胱中，SERCA的蛋白表达的损失是受基因表达下游调控的介导的。这些数据表明平滑肌离子泵的基因表达部分的受机械因素（压力作用）:P调节。







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