[求助]请教高手看下我设计的引物可行不

我开始做毕业论文了，第一次自己设计引物，是用PP5设计的，请各位高手看下有没有问题：
基因组序列：agttcctgat ttcaattcct tattggagct acctcttaga gtttctcact tcattttttt
tttcttccgg gatgtggagt gttttcatcg ttagttggtt tttagatctc tcagattttg
ttttcctttc tacaagaaac tggttatcgg aagagataat taagtttttt tcttttcttt
tcaaaaagag tattttgtga tgatttctaa ctttcaagag atctttgctt atgattgtgt
tttgcttgtc gtatactttt cttcttctaa aatacagcaa aacattgctg tcatcttctt
cttctctttt tttttttttt ttgtgagcat cttcatttaa taaaaaatac acaaagcaat
gctgtcatct gcggatattt ttaagcagat caagatcatt attttctgtt tcctagtgat
agtatctaag gggttttatt acatcagttt cctgtttcac tatgtctttg ttttacagaa
gaataacaat ggctggctct gttaacgggt atcaaactcc cggtagaaat catgtttcgg
tgtctgaagt ggatgacttt tgcattgcac ttggagggaa aaggccaatc catagcattt
tgatcgctaa caatggaatg gcagctgtta agtttatacg cagtgtcaga acatgggctt
acgaaacatt tggcaccgag agagctattt tgttggttgg gatggcgact cctgaagaca
tgaggatcaa tgccgagcat atcagaatcg ctgatcagtt tgtcgaggtt cccggaggaa
ctaacaataa caattatgcc aacgttcagc ttatcgttga ggtgggtgaa tacaaccaat
agtaataata ataatacctc atgaagaagt tttgtatctg ttgagtttaa tcctgaattc
atctttgtct actatgtgca gatggctgaa gtaacacgtg tggatgcagt ttggcctggt
ggggtcatg catctgagaa ccctgagtta cctgatgcgt tgaaggcaaa aggaattata
tttcttggtc ctccagcagc ttcaatggca gcattaggtg acaagattgg ttcctcgttg
attgcacaag ctgctgatgt acctactcta ccatggagtg gatcccatgt aagtaatttt
tgtgtcccgt tacgctgaac ttgtttacta gagaattgtt tcaaaatgac ccaagtttta
其中引物部分用红色标出：
上游：gaagaaactggttatcgaag
下游：tgctggaggaccaagaaata