【求助】求助红色部分的翻译

1.Four protomers pack symmetrically to generate a two-layer architecture: the intracellular cytoplasmic domain (ICD) layer, and the transmembrane domain (***) layer (Fig. 2b, c). The central four-fold rotation axis is perpendicular to both layers. The hTRPC6 ICD has an inverted bell shape and caps below the ion channel pore of ***.

2.个人理解：四个原型对称包装以产生两层结构：细胞内细胞质结构域（ICD）层和跨膜结构域（***）层（图2b，c）。中央四重旋转轴垂直于两个层。 hTRPC6 ICD具有倒置的钟形形状，并且在***的离子通道孔下方具有帽？/hTRPC6 ICD位于***的离子通道孔下方并具有倒置的钟形形状和帽状结构？（还是我的理解都不对，这个caps究竟该如何理解？）

3.语境：上文阐述的是经典瞬时受体电位（canonical transient receptor potential，TRPC）通道6构成四聚体的组装方式，TRPC6通道是一类非选择性阳离子通道，一般组成四聚体发挥作用。

4.摘录一部分便于理解

Architecture of the hTRPC6 channel To stabilize the hTRPC6 channel in closed state, we conducted cryo-electron microscopic (cryo-EM) studies of hTRPC6 in complex with the inhibitor BTDM. The hTRPC6 protein overexpressed in mammalian cells was purified in detergent micelles and reconstituted into nanodiscs for single-particle analysis (Supplementary information, Figures S1f and g and S2). The 3.8 Å cryo-EM map was of sufficient quality for us to model 670 out of the 931 residues (Supplementary information, Figures S3 and S4 and Table S2). The remaining residues were mostly disordered, probably due to their flexibility in the closed state. The hTRPC6 channel tetramer occupies 75 × 75 × 150 Å3 in three−dimensional space (Fig. 2 and Supplementary information, Video S1). Four protomers pack symmetrically to generate a two-layer architecture: the intracellular cytoplasmic domain (ICD) layer, and the transmembrane domain (***) layer (Fig. 2b, c). The central four-fold rotation axis is perpendicular to both layers. The hTRPC6 ICD has an inverted bell shape and caps below the ion channel pore of ***. Its Nterminus (1–84) was unresolved in our cryo-EM density map. Truncation of the N-terminal amino acids 1–71 did not affect the tetramer assembly or OAG-induced depolarization of membrane potential (Supplementary information, Figure S5 and Table S1), indicating that the flexible N terminal 71 residues were dispensable for hTRPC6 assembly and gating. 

5.原文见附件

6. ***是transmembrane domain的缩写，因为该缩写类似于信息敏感被替代了