【求助】我需要分离并鉴定尿中的一种蛋白
另外,检测方法是:Cationic peptides were extracted from urine using CM Macroprep (Bio-Rad, Hercules, CA). Urine extracts equivalent to 4 mg of creatinine were
analyzed along with synthetic hepcidin standards (0.05, 0.15, 0.5, and 1.5μg) by sodium dodecyl sulfate (SDS)–Tricine–polyacrylamide gel electrophoresis (PAGE) and Western blotting. Hepcidin was detected on the blots using rabbit antihuman hepcidin antibody.
这里为什么要用等量的肌酐?有的资料还要用高压色普分析法来纯化,我只是做这种蛋白在不同人体表达量的差异,有这个必要吗?
这些检测方法难吗?(我的这种水平可以做吗?),花费高吗? 谢谢!
急急急!!!
盼复。
rabbit immunization with synthetic hepcidin1 conjugated to keyhole limpet hemocyanin (KLH, no. 7762;Pierce, Rockford, IL).这个抗体我怎么在这家公司网站在找不到呢?好几篇文献都用的这家公司产品。