细胞免疫荧光染色背景高 请问有什么建议吗
发布于 2019-12-01 · 浏览 3696 · IP 山东山东
这个帖子发布于 5 年零 170 天前，其中的信息可能已发生改变或有所发展。
My cells are cultured primary neuron from fetal rat, transfected with GFP-HA-double tag surface protein at DIV 7.
IF was preformed at DIV10-12, fixation with 4% PFA 15min and block with 10% Donkey serum. 594 fluorenes marked HA tag.
below is my picture captured with 60X microscope. It is quite ugly. I am new to this and want to know what happend, would you please help me figure out?
最后编辑于 2022-10-09 · 浏览 3696
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