巴恩斯（Barnes）迷宫实施注意事项（持续更新）

呀呀呀，求关注呀

没人带，和师姐自己摸索出来的。正在做，可能会不停修改。

1.SMART3.0软件操作:

打开软件，选择好摄像头

调整视野:尝试了一下，只能画个方框。

选择区域:因为没有现成的barnes区域可以选，所以我们就手动画圈，不同颜色区分。如整个区域框是红色，目标区域是绿色圆圈，非目标区域是蓝色圆圈。设置绿色圆圈名字和属性为target zone。可以保存为一种自定义的模式，下次就不用再画那么多圈了。

照相和测试:我们用的是小黑鼠，根据工程师建议调低了亮度和对比度（测试房间不关灯）。

之前:选择追踪方式为centre of mass（因为triwase不能用！工程师也不知道为啥，推测是因为我们的小鼠太大！如果能用triwase的话，旁边还可以设置不同方式的追踪。）

后来:工程师来调整了圆的直径，就可以用triswase了，选两点追踪。

接下来的步骤很重要！先不放小鼠照相，注意不要照到人！看一下照的照片没问题后，放小鼠，点测试，有追踪点就可以了。（追踪点是一个红点，旁边会有坐标数据）

设置时间：设置潜伏期时间14s，10s小鼠在中央的盒子里，4s人进去拿走盒子，打开噪音。正式测试时间为4分钟。设置停止方式为进入目标区域后1s。（第二个选项。）

添加测试对象:每做一只小鼠都得新添加一个对象。填好code和分组，然后在session那里，左边选中新添加的对象，然后点》，选择一个session，使trial和subject对应，为绿色剪头说明是即将要做的对象，红色剪头说明是做完了。

开始运行。把握好时间，人尽早出来，不然会计到人。

2.测试流程:

Adaptation period:

1) Place the mouse in a cylindrical black start chamber in the middle of the maze. After 10 s have elapsed lift the chamber, switch the buzzer on and guide the mouse gently to the escape box. Do not force the animal to get into the hole as this may be too stressful. If the mouse does not want to get into the target hole, place it gently to the side of the hole and pull gently the base of the tail in the direction away from the hole, which induces the mouse to move in the opposite direction of the tail pull and enter the hole.If this does not work, place the mouse directly into the escape box.

2) Once the mouse is inside the box, buzzer, light and/or fan should be turned off.

3) The mouse should stay in the escape box for 2 min.

Spatial Acquisition:

4) Before the acquisition phase begins, the maze must be cleaned using a solution with 1% Incidin solution (Incidin extra N, Lahmann Rauscher) to avoid olfactory cues. In addition to cleaning, the maze should be rotated around its central axis after each trial in order to control for possibly remaining odour cues.

5) Place the mouse in the cylindrical black start chamber in the middle of the maze. After 10 s have elapsed lift the chamber, switch the buzzer on and allow the mouse explore the maze for 3 minutes.

6) During these 3 minutes number of primary errors, total errors and primary latency should be measured by the experimenter.

7) The trial will end when the mouse enters the goal tunnel or after 3 min have elapsed. Immediately after the mouse enters the tunnel, the buzzer should be turned off and the mouse is allowed to stay in the tunnel for 1 min.

8) If the mouse does not reach the goal within 3 minutes the experimenter should guide the mouse gently to the escape box (see Step 1) and leave the mouse inside for 1 min.

Mice may go out of the escape box during the time it should stay in it. You may cover the escape hole once the mouse is inside for 1 minute or just place mice gently in the escape box whenever it goes out.

我们做的时候是测试4min，训练时让它在正确的盒子里待30s。

噪音在80分贝左右。

灯一直开着，没关。

一天训练2次，正式测试一次，记最后一次的数据。每次之间间隔不能太长，文献写的15min，我们大概半小时。

3.数据分析:

待更。