【转载】现代组学技术研究中药：川芎嗪抑制小胶质细胞活化的机制研究
发布于 2014-12-19 · 浏览 965 · IP 北京北京
这个帖子发布于 10 年零 148 天前，其中的信息可能已发生改变或有所发展。
转载理由： 现代生命科学的组学技术与中国传统医学的结合，可能会擦出新的火花，以下就是例子：
在世界范围内，神经退行性疾病的发病率和死亡率均居于较高水平，一些研究已经证实川穹嗪（tetramethylpyrazine，TMP）对神经退行性疾病有效，该疗效是通过抑制小胶质细胞对炎症刺激的活化而实现的。但是，TMP作用的分子机制仍然未知。
重庆医科大学的研究人员在近期的研究中，利用蛋白质组学技术从新的角度阐释了TMP的做用机制：研究人员利用iTRAQ技术和 LC TRIPLE-TOF对经过或不经过TMP处理的脂多糖激活的小胶质细胞进行了蛋白质组学分析，鉴定出了5184种蛋白，其中266种具有差异表达，蛋白注释显示了差异蛋白包括iNOS、ERO1L等和降低细胞氧化应激反应相关的蛋白，进一步的Pathway分析表明，差异蛋白主要分布在包括LXR/RXR活化、NO产生等信号通路中。再次基础上，研究人员利用Western-blot进一步验证了iNOS蛋白的差异表达。
该研究进一步表明，iTRAQ技术应用于TMP抑制小胶质细胞活性的分子机制研究是有效的，TMP降低了LXR/RXR介导的iNOS，并由此减少了炎症刺激下小胶质细胞的活化。
iTRAQ-based proteomic analysis of tetramethylpyrazine inhibition on lipopolysaccharide-inducedmicroglial activation.
AbstractAIMS:
Neurodegenerative diseases are the leading cause of morbidity and mortality worldwide. Several studies have shown thattetramethylpyrazine (TMP) is an effective therapy for neurodegenerative diseases and that it acts by inhibiting the activation of microglial cells in response to inflammatory stimuli. However, the molecular mechanisms underlying the action of TMP remain unknown.
MAIN METHODS:
Proteomic analysis was used to generate novel insights into the mechanism by which TMP inhibits microglial activation, and western blotting was used to validate candidate proteins.
KEY FINDINGS:
To identify candidate proteins affected by TMP in lipopolysaccharide-activated microglia, we performed proteomic analysisusing iTRAQ labelling coupled with LC TRIPLE-TOF, and we identified 5187 unique proteins. Among these, 266 proteins were differentially expressed and considered putative candidate proteins. Protein annotation revealed that the differentially expressed proteins, such as inducible nitric oxide synthase (iNOS) and ERO1-like protein (ERO1L), might be involved in reducing cellular oxidation in response to stress. Ingenuity pathway analysis revealed that the differentially expressed proteins were involved in a variety of signalling pathways, including liver X receptor/retinoid X receptor (LXR/RXR) activation and the production of nitric oxide and reactive oxygen species in macrophages. Furthermore, one of the differentially expressed protein candidates detected by iTRAQ, iNOS, was confirmed by western blotting.
SIGNIFICANCE:
Our data suggest that iTRAQ technology is an effective tool to study the mechanism by which TMP inhibits activated microglia. TMP decreased the expression of LXR/RXR-mediated iNOS, which reduced microglial activation in response to inflammatory stimuli.
。
。
转载自： 邦菲iTRAQ论坛
原文链接： http://bbs.itraq.cn/forum.php?mod=viewthread&tid=5920&extra=
在世界范围内，神经退行性疾病的发病率和死亡率均居于较高水平，一些研究已经证实川穹嗪（tetramethylpyrazine，TMP）对神经退行性疾病有效，该疗效是通过抑制小胶质细胞对炎症刺激的活化而实现的。但是，TMP作用的分子机制仍然未知。
重庆医科大学的研究人员在近期的研究中，利用蛋白质组学技术从新的角度阐释了TMP的做用机制：研究人员利用iTRAQ技术和 LC TRIPLE-TOF对经过或不经过TMP处理的脂多糖激活的小胶质细胞进行了蛋白质组学分析，鉴定出了5184种蛋白，其中266种具有差异表达，蛋白注释显示了差异蛋白包括iNOS、ERO1L等和降低细胞氧化应激反应相关的蛋白，进一步的Pathway分析表明，差异蛋白主要分布在包括LXR/RXR活化、NO产生等信号通路中。再次基础上，研究人员利用Western-blot进一步验证了iNOS蛋白的差异表达。
该研究进一步表明，iTRAQ技术应用于TMP抑制小胶质细胞活性的分子机制研究是有效的，TMP降低了LXR/RXR介导的iNOS，并由此减少了炎症刺激下小胶质细胞的活化。
iTRAQ-based proteomic analysis of tetramethylpyrazine inhibition on lipopolysaccharide-inducedmicroglial activation.
AbstractAIMS:
Neurodegenerative diseases are the leading cause of morbidity and mortality worldwide. Several studies have shown thattetramethylpyrazine (TMP) is an effective therapy for neurodegenerative diseases and that it acts by inhibiting the activation of microglial cells in response to inflammatory stimuli. However, the molecular mechanisms underlying the action of TMP remain unknown.
MAIN METHODS:
Proteomic analysis was used to generate novel insights into the mechanism by which TMP inhibits microglial activation, and western blotting was used to validate candidate proteins.
KEY FINDINGS:
To identify candidate proteins affected by TMP in lipopolysaccharide-activated microglia, we performed proteomic analysisusing iTRAQ labelling coupled with LC TRIPLE-TOF, and we identified 5187 unique proteins. Among these, 266 proteins were differentially expressed and considered putative candidate proteins. Protein annotation revealed that the differentially expressed proteins, such as inducible nitric oxide synthase (iNOS) and ERO1-like protein (ERO1L), might be involved in reducing cellular oxidation in response to stress. Ingenuity pathway analysis revealed that the differentially expressed proteins were involved in a variety of signalling pathways, including liver X receptor/retinoid X receptor (LXR/RXR) activation and the production of nitric oxide and reactive oxygen species in macrophages. Furthermore, one of the differentially expressed protein candidates detected by iTRAQ, iNOS, was confirmed by western blotting.
SIGNIFICANCE:
Our data suggest that iTRAQ technology is an effective tool to study the mechanism by which TMP inhibits activated microglia. TMP decreased the expression of LXR/RXR-mediated iNOS, which reduced microglial activation in response to inflammatory stimuli.
。
。
转载自： 邦菲iTRAQ论坛
原文链接： http://bbs.itraq.cn/forum.php?mod=viewthread&tid=5920&extra=
最后编辑于 2014-12-19 · 浏览 965
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