【求助】请教一个膜片钳操作以及数据统计的问题
这个帖子发布于 18 年零 336 天前，其中的信息可能已发生改变或有所发展。
这是一篇关于膜片钳记录NMDA电流的文献，具体方法如下：
N-meth¬yl-D-aspartate (3–1000 μM) was applied for eight sec from the array of the pipe 100 μm from the cells, the interval between applications was 1.5 min. Next, 50 μM AP5 and 3–1000 μM NMDA were applied to confirm the inward currents mediated by NMDA receptors. The concentration in the effect compart¬ment related to 50% effect (EC50) value of NMDA was achieved according to the Hill equation. In most neurons, the desensitization of NMDA-gated currents reached steady state after a four second application of NMDA. In this study; steady state NMDA-gated currents were considered the amplitude of currents at the end of eight second exposure of different concen¬trations of NMDA to confirm NMDA-gated currents reached steady state in all neurons.
随后得到如图的结果，有点疑惑的是：
1.上文说每次给药8秒钟，但是电流的时程似乎也不到10秒，是不是说一停止给药，细胞的电流就会消失呢，我自己的经验好像不是这样（文献中用的是急性分离细胞）
2.附图中的统计表中的Normalized currents该怎么理解？
谢谢
N-meth¬yl-D-aspartate (3–1000 μM) was applied for eight sec from the array of the pipe 100 μm from the cells, the interval between applications was 1.5 min. Next, 50 μM AP5 and 3–1000 μM NMDA were applied to confirm the inward currents mediated by NMDA receptors. The concentration in the effect compart¬ment related to 50% effect (EC50) value of NMDA was achieved according to the Hill equation. In most neurons, the desensitization of NMDA-gated currents reached steady state after a four second application of NMDA. In this study; steady state NMDA-gated currents were considered the amplitude of currents at the end of eight second exposure of different concen¬trations of NMDA to confirm NMDA-gated currents reached steady state in all neurons.
随后得到如图的结果，有点疑惑的是：
1.上文说每次给药8秒钟，但是电流的时程似乎也不到10秒，是不是说一停止给药，细胞的电流就会消失呢，我自己的经验好像不是这样（文献中用的是急性分离细胞）
2.附图中的统计表中的Normalized currents该怎么理解？
谢谢
4 1 点赞
