做细胞培养时96孔板中的液体如何同时搅拌？

大家好，我初次做细胞实验，身边无人指导，有疑问想请教下做过的朋友们。

问题如下：

我对下面过程中的 wash ，remove，agitate，Spin操作不知怎么做。

wash是洗涤的意思，那96孔板上生长的贴壁细胞如何同时洗呢？肯定不能用移液*同时吹打吧？

remove是移除的意思，那96孔板上的孔内液体应该如何同时移除呢？

agitate是搅拌的意思，那96孔板上孔内的液体如何同时搅拌呢？

可能我本身理解有误，还是这些操作需要借助某个仪器才能实现？

文献上的英文操作过程如下，请各位做过这类实验的朋友指导下，十分感谢。

1) Cells were placed in the Poly-D-lysine 96 well microplates at the density of 6*10>4 /well the day before the assay. 

2) At least 12 h after the cells plated, wash the cells three times with 200μl/well of pre-warmed Cl- free HBSS Buffer, and remove all buffers at last washing. 

3) Add 50μl per well of Cl- free HBSS Buffer plus Uric acid [8-14C] (0.1μCi/well) with or without Benzbromarone or tested compounds to the cells incubated for 5 minutes at 37 ℃. 

4) At the end of 5 minutes incubation, Uric acid [8-14C] uptake was stopped by removing the incubation buffer and adding 100μl of ice-cold Cl- free HBSS Buffer. 

5) Wash three times with this solution and clean the buffer in the wells. 

6) Add 50μl per well lysis buffer to lysate cells, and agitate at 600 rmp for 10 min. 

7)Spin the plate at 1000 rmp for 5 min and remove 45μl of supernatant to Isoplate-96 Microplate. 

8) Add 150μl per well Ultima GoldTM XR scintillation cocktail and agitate at 600 rmp for 10 min.