[Science Express] 线虫的大规模蛋白相互作用数据

A Map of the Interactome Network of the Metazoan C. elegans

Published online January 2, 2004

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput yeast two-hybrid (Y2H) screens. Independent co-affinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ~5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

http://www.sciencemag.org/cgi/content/abstract/1091403v1

这是继2000年前后对于budding yeast(出芽酵母)/幽门螺杆菌和2003年10月对于果蝇之后，又一次的对于模式生物:线虫(C.elegans)的蛋白质相互作用进行大规模的测定！以哈福大学牵头20几家单位参与共同完成；可惜主要还是使用酵母双杂交（Y2H）的方法，(这种方法的假阳性太高),但是文章中说他们独立地使用了共亲和纯化的实验方法(Independent co-affinity purification assays )对于全部数据进行了的验证！相信可靠度是很高的；目前线虫相互作用组(the Worm Interactome (WI5) map)含有相互数据~5500,其中可能包括部分预测数据！文章对于这套数据形成的相互作用网络进行了拓扑性质和生物学性质的分析研究！

这套数据给比较生物学和蛋白相互作用研究提供了一个好机会！