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想请教一下做PCR这方面的大佬，用的是taqman探针进行的PCR曲线非常奇怪，（已经采用SYBR green法验证过了引物，模板，没有问题，两种方法用的是同一管逆转录产物），第一个图是染料法，第二个图是探针法，不知道是探针设计的不好还是怎么回事?跪求大佬解答

PCR 探针法扩增曲线异常问题

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