WB 检测磷酸化水平，37度水浴的目的是什么？谢谢

WB检测CAR-T细胞的磷酸化水平， 为什么要在37度分别水浴5 min，30min和60min？

4Ig-B7-H3 fused with mouse IgG2a Fc (Chimerigen Laboratories) for 20 minutes and then cross-linked with 2 μL of goat anti-mouse secondary Ab (BD Bioscience) for an additional 20 minutes. Cells were then transferred to a 37 °C water bath for the indicated time points and lysed with 2 × Laemelli Buffer.