【摘要翻译】非洲裔美国人前列腺癌患者中P27和SKP2蛋白表达的改变
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非洲裔美国人前列腺癌患者中P27和SKP2蛋白表达的改变
摘要:
目的:
本试验的目的在于揭示非洲裔美国人前列腺原位癌患者中P27和SKP2蛋白表达的改变与临床改变的相关性. P27(一种细胞增殖抑制因子)的含量受SKP2依赖的蛋白水解作用的控制.
方法：
将一组具有良好代表性,曾经于1999-2000年在纽约Veterans affairs 医学中心做过前列腺根治性切除术的162名非洲裔美国人作为研究对象.作者使用免疫组化方法分析P27和SKP2蛋白表达,试图分析P27(<40%的肿瘤细胞表达这种蛋白)和SKP2(>=20%的肿瘤细胞表达这种蛋白)与临床病理参数和前列腺特异性抗原重现时间的相关性.
结果:
P27和SKP2蛋白表达的改变分别在162名病例的112名(69.1%),93名(57.4%)中被发现 。 在87名(53.7%)患者中发现P27和SKP2蛋白表达呈现相反趋势.在SKP2蛋白过表达和前列腺癌囊外浸润之间有较显著的相关性(P=0.065).而且, SKP2蛋白过表达患者相对于低表达者,其PSA的重现平均推迟了2.77年.但是,这种差异无统计学显著性.在多因素分析中,只有肿瘤的分级和分期可以预测患者的PSA重现时间.
结论:
这些资料表明在前列腺癌的发病机制中SKP2蛋白过表达可能与P27的降解没有严格的相关性.需要更多的研究来确定SKP2在前列腺癌中的作用机制.
原文:
Altered expression of p27 and Skp2 proteins in prostate cancer of African-American patients.
Drobnjak M, Melamed J, Taneja S, Melzer K, Wieczorek R, Levinson B, Zeleniuch-Jacquotte A, Polsky D, Ferrara J, Perez-Soler R, Cordon-Cardo C, Pagano M, Osman I.
Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
PURPOSE: The purpose is to investigate the clinical relevance of altered patterns of p27 and Skp2 expression in African-American patients with localized prostate cancer. The abundance of p27, an inhibitor of cell proliferation, is controlled by Skp2-dependent proteolysis. EXPERIMENTAL DESIGN: A well-characterized cohort of 162 African-Americans who underwent radical prostatectomy at the Veterans Affairs Medical Center of New York between 1990 and 2000 was studied. We analyzed p27 and Skp2 expression by immunohistochemistry. Altered expression of p27 (defined as <40% tumor cells expressing the protein) and Skp2 (defined as > or ==" BORDER="0">20% tumor cells expressing the protein) were correlated with clinicopathological parameters and time to prostate-specific antigen (PSA) recurrence. RESULTS: Altered expression of p27 and Skp2 was observed in 112 of 162 (69.1%) and 93 of 162 (57.4%) cases, respectively. Inverse patterns of Skp2 and p27 protein expression were seen in 87 of 162 (53.7%) cases. A marginally significant association was found between Skp2 overexpression and extracapsular extension (P = 0.065). Moreover, patients with Skp2 overexpression had a 2.77 years decreased median time to PSA recurrence compared with patients with low Skp2 expression; however, the difference was not statistically significant. In multivariate analysis, only tumor grade and stage independently predicted PSA recurrence in this cohort. CONCLUSIONS: Our data suggest a role for Skp2 overexpression in prostate cancer pathogenesis that might not be exclusively related to p27 degradation. More studies are needed to determine the mechanistic role of Skp2 in prostate cancer.
摘要:
目的:
本试验的目的在于揭示非洲裔美国人前列腺原位癌患者中P27和SKP2蛋白表达的改变与临床改变的相关性. P27(一种细胞增殖抑制因子)的含量受SKP2依赖的蛋白水解作用的控制.
方法：
将一组具有良好代表性,曾经于1999-2000年在纽约Veterans affairs 医学中心做过前列腺根治性切除术的162名非洲裔美国人作为研究对象.作者使用免疫组化方法分析P27和SKP2蛋白表达,试图分析P27(<40%的肿瘤细胞表达这种蛋白)和SKP2(>=20%的肿瘤细胞表达这种蛋白)与临床病理参数和前列腺特异性抗原重现时间的相关性.
结果:
P27和SKP2蛋白表达的改变分别在162名病例的112名(69.1%),93名(57.4%)中被发现 。 在87名(53.7%)患者中发现P27和SKP2蛋白表达呈现相反趋势.在SKP2蛋白过表达和前列腺癌囊外浸润之间有较显著的相关性(P=0.065).而且, SKP2蛋白过表达患者相对于低表达者,其PSA的重现平均推迟了2.77年.但是,这种差异无统计学显著性.在多因素分析中,只有肿瘤的分级和分期可以预测患者的PSA重现时间.
结论:
这些资料表明在前列腺癌的发病机制中SKP2蛋白过表达可能与P27的降解没有严格的相关性.需要更多的研究来确定SKP2在前列腺癌中的作用机制.
原文:
Altered expression of p27 and Skp2 proteins in prostate cancer of African-American patients.
Drobnjak M, Melamed J, Taneja S, Melzer K, Wieczorek R, Levinson B, Zeleniuch-Jacquotte A, Polsky D, Ferrara J, Perez-Soler R, Cordon-Cardo C, Pagano M, Osman I.
Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
PURPOSE: The purpose is to investigate the clinical relevance of altered patterns of p27 and Skp2 expression in African-American patients with localized prostate cancer. The abundance of p27, an inhibitor of cell proliferation, is controlled by Skp2-dependent proteolysis. EXPERIMENTAL DESIGN: A well-characterized cohort of 162 African-Americans who underwent radical prostatectomy at the Veterans Affairs Medical Center of New York between 1990 and 2000 was studied. We analyzed p27 and Skp2 expression by immunohistochemistry. Altered expression of p27 (defined as <40% tumor cells expressing the protein) and Skp2 (defined as > or ==" BORDER="0">20% tumor cells expressing the protein) were correlated with clinicopathological parameters and time to prostate-specific antigen (PSA) recurrence. RESULTS: Altered expression of p27 and Skp2 was observed in 112 of 162 (69.1%) and 93 of 162 (57.4%) cases, respectively. Inverse patterns of Skp2 and p27 protein expression were seen in 87 of 162 (53.7%) cases. A marginally significant association was found between Skp2 overexpression and extracapsular extension (P = 0.065). Moreover, patients with Skp2 overexpression had a 2.77 years decreased median time to PSA recurrence compared with patients with low Skp2 expression; however, the difference was not statistically significant. In multivariate analysis, only tumor grade and stage independently predicted PSA recurrence in this cohort. CONCLUSIONS: Our data suggest a role for Skp2 overexpression in prostate cancer pathogenesis that might not be exclusively related to p27 degradation. More studies are needed to determine the mechanistic role of Skp2 in prostate cancer.
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