【求助】有人用结晶紫染色法测定细胞增殖活性的吗？

最近看到很多国外的文献在做细胞增殖活性的时候，采用的是crystal violet（结晶紫）染色法，而不是用我们经常采用的MTT法，从步骤来看，不比MTT法复杂，原理上也差不多，也是利用活细胞被结晶紫染色后形成结晶，SDS溶解结晶后测OD值来反映细胞数量。但是文献却说这种方法比MTT法稳定，重复性好，不知道有没有战友做过这个实验，能不能介绍介绍经验，是不是真的比MTT法重复性要好？
为了便于说明，把我找到的一个crystal violet染色的protocol贴上，供大家对比讨论：
Crystal Violet Assay
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay, crystal violet, stains DNA. Upon solubilization, the amount of dye taken up by the monolayer can be quantitated in a spectrophotometer or plate reader.

Carefully remove culture medium from wells.
Wash plate gently with PBS warmed at least to room temperature: Number of wells Volume：
96 0.2 mL
48 0.5 mL
24 1 mL
12 2 mL
6 3 mL

Carefully remove PBS and add crystal violet solution. Incubate 10 minutes at room temperature: Number of wells Volume：
96 50 uL
48 100 uL
24 200 uL
12 500 uL
6 750 uL

Wash plate 2x in tap water by immersion in a large beaker. Be careful not to lift off cells. Change tap water between washes.

Drain upside down on paper towels, than add 1% SDS to solubilize the stain: Number of wells Volume：
96 100 uL
48 300 uL
24 600 uL
12 1 mL
6 1.5 mL

Agitate plate on orbital shaker until color is uniform with no areas of dense coloration in bottom of wells.
Read absorbence of each well at 570 nm.