分享书籍 heterologous protein production in CHO cells(Methods and Protocols) 2017

分享书籍一本，以下为该书目录：

Preface..........................................................  v

Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix

1  Strategies and Considerations for Improving Expression of “Difficult 

to Express” Proteins in CHO Cells.................................  1

Christina S. Alves and Terrence M. Dobrowsky

2  Glycoengineering of CHO Cells to Improve Product Quality.............  25

Qiong Wang, Bojiao Yin, Cheng-Yu Chung, and Michael J. Betenbaugh

3  Large-Scale Transient Transfection of Chinese Hamster Ovary Cells 

in Suspension.................................................  45

Yashas Rajendra, Sowmya Balasubramanian, and David L. Hacker

4  Cloning of Single-Chain Antibody Variants by Overlap- Extension PCR 

for Evaluation of Antibody Expression in Transient Gene Expression........  57

Patrick Mayrhofer and Renate Kunert

5  Anti-Apoptosis Engineering for Improved Protein Production 

from CHO Cells...............................................  71

Eric Baek, Soo Min Noh, and Gyun Min Lee

6  Conditional Knockdown of Endogenous MicroRNAs in CHO Cells 

Using TET-ON-SanDISponge Vectors.............................  87

Alan Costello, Nga Lao, Martin Clynes, and Niall Barron

7  Application of CRISPR/Cas9 Genome Editing to Improve 

Recombinant Protein Production in CHO Cells.......................  101

Lise Marie Grav, Karen Julie la Cour Karottki, Jae Seong Lee, 

and Helene Faustrup Kildegaard

8  Improved CHO Cell Line Stability and Recombinant Protein Expression 

During Long-Term Culture......................................  119

Zeynep Betts and Alan J. Dickson

9  Selection of High-Producing Clones Using FACS for CHO 

Cell Line Development..........................................  143

Clair Gallagher and Paul S. Kelly

10  The ‘Omics Revolution in CHO Biology: Roadmap to Improved 

CHO Productivity.............................................  153

Hussain Dahodwala and Susan T. Sharfstein

11  A Bioinformatics Pipeline for the Identification of CHO Cell Differential 

Gene Expression from RNA-Seq Data...............................  169

Craig Monger, Krishna Motheramgari, John McSharry, Niall Barron, 

and Colin Clarke

12  Filter-Aided Sample Preparation (FASP) for Improved Proteome 

Analysis of Recombinant Chinese Hamster Ovary Cells..................  187

Orla Coleman, Michael Henry, Martin Clynes, and Paula Meleady

13  Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the 

Phosphoproteome of Recombinant Chinese Hamster Ovary Cells..........  195

Michael Henry, Orla Coleman, Prashant, Martin Clynes, 

and Paula Meleady

14  Engineer Medium and Feed for Modulating N-Glycosylation 

of Recombinant Protein Production in CHO Cell Culture...............  209

Yuzhou Fan, Helene Faustrup Kildegaard, and Mikael Rørdam Andersen

15  Glycosylation Analysis of Therapeutic Glycoproteins Produced 

in CHO Cells.................................................  227

Sara Carillo, Stefan Mittermayr, Amy Farrell, Simone Albrecht, 

and Jonathan Bones

16  Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses...  243

Catherine E.M. Hogwood, Lesley M. Chiverton, and C. Mark Smales

Index  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251