请教在NCBI的BLAST里检测引物的结果怎么看？
发布于 2004-09-06 · 浏览 1.6 万 · IP 新疆新疆
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gi|52663|emb|X01772.1|MMIL04 Mouse mRNA for interleukin-2 (IL-2)
Length = 939
Score = 48.1 bits (24), Expect = 0.001
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 1 attacaggaacctgaaactcccca 24
||||||||||||||||||||||||
Sbjct: 238 attacaggaacctgaaactcccca 261
Score = 46.1 bits (23), Expect = 0.004
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 25 tgagggcttgttgagatgatgct 47
|||||||||||||||||||||||
Sbjct: 553 tgagggcttgttgagatgatgct 531
这不是说明我的引物很好吗，为什么我扩不出来呢？
Length = 939
Score = 48.1 bits (24), Expect = 0.001
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 1 attacaggaacctgaaactcccca 24
||||||||||||||||||||||||
Sbjct: 238 attacaggaacctgaaactcccca 261
Score = 46.1 bits (23), Expect = 0.004
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 25 tgagggcttgttgagatgatgct 47
|||||||||||||||||||||||
Sbjct: 553 tgagggcttgttgagatgatgct 531
这不是说明我的引物很好吗，为什么我扩不出来呢？
最后编辑于 2022-10-09 · 浏览 1.6 万
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