我得第一篇摘要（牙周专业）：急盼那宝贵的第一分

文题: Are there HLA combinations typical supporting for or making resistant against aggressive and/or chronic periodontitis?
A作者: Stein,-J; Reichert,-S; Gautsch,-A; Machulla,-H-K
杂志全名: Journal of Periodontal Research.
年份，卷（期）: 起止页码：Journal of Periodontal Research 2003 Oct; 38(5): 508-17
PMID：12941076
英文摘要: OBJECTIVE AND BACKGROUND: Human leukocyte antigens (HLA)/alleles have been considered as risk factors for periodontal disease. However, data from HLA associations is not consistent. Diversity of HLA antigen combinations and en bloc inherited HLA alleles (haplotypes), as known in systemic diseases, can be variable factors in disease association. Therefore, the aim of this study was to investigate the incidence of HLA homozygosities, heterozygosities and estimated haplotypes in German Caucasian groups with generalized aggressive (N = 50) and chronic (N = 102) periodontitis in comparison to control probands without periodontitis (N = 102). METHODS: HLA-A, -B, -Cw, -DRB1, -DRB3/4/5, -DQB1 typing was carried out using both serologic (microlymphocytotoxicity test) and genomic (PCR-SSP: PCR with sequence specific primers) techniques. Frequencies of all homozygosities, heterozygosities and haplotypes were determined in all patients and controls. RESULTS: In both patient groups, associations to HLA homozygosities and heterozygosities were found. Most striking was the significantly lower frequency of HLA-DRBblank* homozygosity (non-DRB3*/DRB4*/DRB5*) in chronic periodontitis (p < 0.05), whereas HLA-DRB1*15 : DRB5*(DR51) : DQB1*06 showed a slightly higher homozygosity rate in all patients. As the combination HLA-A*02,A*03 was significantly decreased in aggressive periodontitis (p < 0.05), HLA-A*01,A*03 heterozygosity was significantly lowered in chronic periodontitis (p < 0.05). Among others, the known positive associations for HLA-A*68/69 (A28) and HLA-DRB1*04 were confirmed by the haplotypes HLA-A*68/69 : Cw*07 : B*18 in aggressive periodontitis (p < 0.05) and HLA-Cw*08 : B*14 : DRB1*04 in chronic periodontitis (p < 0.05). CONCLUSION: The present study elucidates the variety of HLA associations and therefore the difficulty to assign single HLA markers to periodontal disease. Susceptibility/resistance of both aggressive and chronic periodontitis may rather be influenced by particular HLA marker combinations. Associated HLA haplotypes may be of further importance for unknown gene loci representing a part of the genetic background for periodontitis. The different associations in aggressive and chronic periodontitis indicate different susceptibility/resistance factors for both diseases.
中文摘要：
目的和背景：已经公认人类白细胞抗原（HLA）/等位基因是牙周疾病的危险因素。然而，关于HLA相关性的研究数据结果并不稳定。在系统性疾病中发现，HLA基因合并的差异和HLA等位基因的全部集合体（单体型）是疾病相关的变量致病因素。因此，本研究目的在于调查HLA基因的纯合性和杂合性发生几率，并评价患侵袭性牙周炎（50例）和慢性牙周炎（102例）的德国高加索人群中HLA单体型与未患牙周炎对照组人群（102例）的差异。
方法：HLA-A, -B, -Cw, -DRB1, -DRB3/4/5, -DQB1型基因应用血清学（微量淋巴细胞毒性试验）和基因学(PCR-SSP:)技术检测。检测所有患者和对照组的HLA基因纯合子，杂合子和单体型的出现几率。
结果：在两组患者组中，发现疾病与HLA的纯合性和杂合性相关。更惊人的是：HLA-DRBblank*纯合子在慢性牙周炎患者中出现几率明显低于其它组（p<0.05）。然而， HLA-DRB1*15 : DRB5*(DR51) : DQB1*06 在所有患者中显示纯合子出现几率的轻度升高。在侵袭性牙周炎患者中HLA-A*02,A*03水平明显降低（p<0.05）， HLA-A*01,A*03 杂合子水平较慢性牙周炎患者低（p<0.05）。 除此之外，在侵袭性牙周炎中已知的HLA-A*68/69 (A28) 和HLA-DRB1*04阳性相关性在侵袭性牙周炎中被HLA-A*68/69 : Cw*07 : B*18单体型证实(p < 0.05)而在慢性牙周炎中被HLA-Cw*08 : B*14 : DRB1*04单体型证实(p < 0.05)
结论：本研究阐明了HLA相关性的多样性，因此很难将牙周病归于由单一的HLA标记物引起。在牙周炎中，相关的HLA单体型对于代表某一基因背景的未知的基因位点更为重要。在侵袭性牙周炎和成人牙周炎忠HLA的不同相关性显示两种疾病的不同易感性/抵抗因素。