凝血酶单位换算

经常有咨询关于凝血酶单位换算问题，这是我在网上查询结果总结出来的，供大家参考：

（1）BP和U没有关联，没有转换关系（BP是按英国药典标定，U是国际单位），两个检测方法不同，用于不同实验，没有关联

（2）凝血酶分子量M=37KDa，根据n=m /M，和各公司凝血酶产品比活，可以计算出mol

（3）1 WHO单位（世界卫生组织单位）

= 1 NIH单位（美国国立卫生研究院单位）

= 1 USP单位（美国药典单位）

= 1.1-1.3 IU（或U）单位（国际单位）

（4）高纯度凝血酶比活大于2000U/mg凝血酶，1mg凝血酶≈2200U≈0.027mmol

参考出处：

①Sigma牛血浆凝血酶说明书（货号：T7513）

②The current International Standard (IS) for alpha-thrombin was established in 1991. It was related in unitage with the 1st IS for Thrombin established in 1975 and contains 100 IU of thrombin activity. The National Institute of Health (NIH) standard (Lot J) is in common use for the calibration of commercial thrombin reagents and this study reports on a comparison of the thrombin units defined by these 2 standards. This study has indicated that one "NIH" unit is equivalent to 1.1 to 1.3 IU of thrombin, depending on the influence of PEG in the assay. A unitage ratio figure of 1.15 was recommended following analysis of data obtained in the presence and absence of PEG in the comparative assays. This was confirmed by amidolytic assay data.

fulltext:Gaffney PJ, Edgell TA. The International and "NIH" units for thrombin--how do they compare? Thromb Haemost. 1995 Sep;74(3):900-3.