急性髓系白血病癌症基因检测

xGen® Acute Myeloid Leukemia Cancer Panel

 xGen® AML Cancer Panel v1.0包含11,743条xGen  Lockdown® Probes,涵盖1.19Mb人类基因，目标富集区域包含超过260个有关AML的基因，可提供更有效的致病突变的检测。

特点：

·探针单独合成与质控

·11,743条xGen® Lockdown Probes锁定的区域内包含260个基因

·通过对200个AML病人进行全基因组与外显子测序定义目标区域

·适用于IDT优化过的xGen4小时捕获Protocol

xGen® Acute Myeloid Leukemia Cancer Panel包含多基因

Table 1. Frequently Researched Genes Included in the xGen® AML Cancer Panel. The panel does not target every exon in each gene, but targets all regions found to be mutated in the TCGA study. For a complete list of genes.

高度的覆盖均一性

Figure 1. Excellent Coverage Uniformity Obtained with xGen® Lockdown AML Cancer Panel. Greater than 0.2 x mean coverage is observed for >98% of targets. Libraries were prepared using the Illumina TruSeq® LT chemistry and sequenced on a MiSeq system using 250 x 250 paired-end reads. A cumulative of 31.8M reads was generated for all four samples.

高覆盖率提高测序通量与领灵敏度

Figure 2. Deep Coverage of Targeted Regions Using xGen® AML Cancer Panel. Four TruSeq® genomic DNA libraries were enriched using the xGen® Acute Myeloid Leukemia Cancer Panel and sequenced on a MiSeq system using 250 x 250 paired-end reads. A total of 31.8M reads were generated. In all samples, there was >1X coverage for 99.8% of targets and >30X coverage for 99.2–99.4% of targets.

高重复率获得一致的结果

Figure 3. Excellent Reproducibility Obtained with xGen® AML Cancer Panel. Multiplexed samples prepared with Illumina TruSeq® libraries were sequenced on the MiSeq sequencing platform using 250 x 250 paired-end reads. A comparison of probe-by-probe target coverage between two samples showed excellent reproducibility, with an R2 value of 0.9831.

 文献

Cancer Genome Atlas Research Network (2013) Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia. N Engl J Med, 368(22):2059–2074