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  Resource index

       About biowww.net

  Molecular biology

       Molecular Cloning: A Laboratory Manual

       Bacterial plasmid bacterialphages

           Molecular Cloning: A Laboratory Manual

           GENE TARGETING VECTORS (Dr. Chris Patzy)

       DNA and Sourthern Blot

           Molecular Cloning: A Laboratory Manual

           Southern and Northern Blot interpretation problem

           Collection of general Southern Blot protocols

           Alkaline Southern Blotting

           Southern Blot Protocol

           Southern Blotting: Detection via Chemiluminescence (BM's Genius System)

           Odyssey Southern Blotting Methods and Troubleshooting Guide

           The Pinpoint Slide DNA Isolation protocol

           Troubleshooting DNA agarose gel electrophoresis

           DNA RNA Protein Tri isolation method (MRC)

           DNA sequencing troubleshooting from Rockefeller DNA sequencing center

       Transfection and infection

           PROCEDURE FOR TRANSFECTION OF MAMMALIAN CELLS USING LIPOFECTAMINE REAGENT

           Molecular Cloning: A Laboratory Manual

           NEW rAVE transfection reagents from GeneDetect

           Retroviral gene transfer and expression mannual

           Promega transfection mannual

           The Qiagen transfection resource book

           Tutorials - Retroviruses and their Applications

           Genetic Screens in Mammalian Cells using Retroviruses

           Troubleshooting and Optimization of Retroviral Transduction Parameters

       DNA-protein interactions

           Molecular Cloning: A Laboratory Manual

       Gene targeting

           ES Cell Culture and Manipulation

           Gene targeting protocols from practical protocol online

           Molecular Cloning: A Laboratory Manual

           Instruction Manual for Gene Targeting

           An in vitro test for assessing the functionality of loxP sites in DNA constructs

           C. elegans Gene Knockout Protocols

           Protocol of transgene construct and ES cell prepartion

           Gene Knockout/Targeting Information Resources 2000 The Scientist Inc.

           Gene Targeting Outline from U. Michigan

           GENE TARGETING VECTORS (Dr. Chris Patzy)

       Glycoconjugates

           Molecular Cloning: A Laboratory Manual

       Microarrays

           DNA microarray protocols

           Fluorescent cDNA microarray protocol

           Molecular Cloning: A Laboratory Manual

           Microarray reviews and papers

           DNA Microarray--- Monitoring the Genome on a Chip

       Mutagenesis

           Targeted Amplification of Mutant Strand (TAMS)

           Unidirectional Deletions (erase-a-base)

           Molecular Cloning: A Laboratory Manual

           Site-directed mutagenesis

           PCR mutagenesis Protocol

           Dut/ung in vitro Site-directed Mutagenesis Protocol

       Protein analysis and western blot

           Molecular Cloning: A Laboratory Manual

           Western blot troubleshooting

           Problem with high molecular weight protein western blot

           Western Blotting with Biotinylated Antibodies

           Western Blotting with Alkaline Phosphatase Conjugates

           Troubleshooting tips of IP, western and immunostaining

           GST Fusion Protein Purification from Yeast

           Introduction to antibodies - Tech handbook from Chemicon

           DNA RNA Protein Tri isolation method (MRC)

       Protein expression

           Problem in purification of the his-tag protein

           Molecular Cloning: A Laboratory Manual

       Protein interactions

           Molecular Cloning: A Laboratory Manual

           Immunoprecipitation With Soluble Antibodies

           Troubleshooting tips of IP, western and immunostaining

       Protein phosphorylation

           Molecular Cloning: A Laboratory Manual

       Cloning and sequencing

           Addition of linkers/adaptors to blunt-end DNA fragments

           Library Screening using PCR

           Screening a cDNA Library

           cDNA Library Screening Protocol - ZAPII

           Molecular Cloning: A Laboratory Manual

           RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING

           Genomic cloning troubleshooting

           Three DNA fragment ligation problems

           PCR products cloning troubleshooting

           Site-directed mutagenesis

           Instruction Manual for Gene Targeting

           An in vitro test for assessing the functionality of loxP sites in DNA constructs

           DNA sequencing troubleshooting from Rockefeller DNA sequencing center

       Reporter gene assay

           Molecular Cloning: A Laboratory Manual

       RNA purification and analysis

           RNA denaturing Gel

           RNA Isolation II (for small amount of tissue)

           Extraction and Purification of Eukaryotic RNA

           how to make nuclease free water

           RNA ELECTROPHORESIS

           Differential Display technical notes

           Molecular Cloning: A Laboratory Manual

           Alternative splicing of mRNA animation tutorial

           Southern and Northern Blot interpretation problem

           DNA RNA Protein Tri isolation method (MRC)

       Yeast

           Molecular Cloning: A Laboratory Manual

           Links to Yeast Protocols on the Web

           Yeast Protein Purification in 96-Well Format

           RNeasy Midi/Maxi Protocols for Isolation of Total RNA

           GST Fusion Protein Purification from Yeast

           Frozen-EZ Yeast Transformation II: Full Protocol

           Frozen Yeast TRAFO Protocol

           Yeast Cell Cycle by Flow Cytometry

           GeneReleaserTM YEAST PROTOCOL

           Immunoprecipitation of Chromatin from Fixed Yeast Protocol

       PCR related

           Targeted Amplification of Mutant Strand (TAMS)

           PCR PRIMER DESIGN AND REACTION OPTIMISATION

           PCR directly from eukaryotic cell without DNA extraction

           PCR Methods from Alkami Biosystems

           PCR trouble shooting from Alkami Biosys

           PCR trouble shooting, help, suggestions

           Primer dimer mystery

           Molecular Cloning: A Laboratory Manual

           Parraffin embeded tissue PCR

           PCR products cloning troubleshooting

           Site-directed mutagenesis

           PCR mutagenesis Protocol

           The Pinpoint Slide DNA Isolation protocol

  Immunology

       Human immunogenetics and immunobiology

       Antibody

           Immunocytochemistry resources collection

           Immunoprecipitation With Soluble Antibodies

           Introduction to antibodies - Tech handbook from Chemicon

       Cytokine Bioassays

           Immunocytochemistry resources collection

       ELISA

           Enzyme-Linked Immunosorbent Assay (ELISA)

           Introduction to antibodies - Tech handbook from Chemicon

       Flow cytometry analysis

           Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis

           Yeast Cell Cycle by Flow Cytometry

           Flow cytometry protocols

       Immunocytochemistry

           Immunocytochemistry resources collection

           Successful Electron Microscopy Immunolabeling

           Whole mount immunocytochemistry

           Dual-immunocytochemistry at the light microscopic level to detect two antigens in the same cell

           Troubleshooting tips of IP, western and immunostaining

           Introduction to antibodies - Tech handbook from Chemicon

       Immunofluorescence

           Immunocytochemistry resources collection

           Troubleshooting nonspecific immunofluoresent labeling

           Staining Nucleic Acids with Propidium Iodide

           Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis

           Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis

           Fluorochrome Absorption and Emission Spectra

           Immunofluorescent Staining of Mouse and Rat Leukocytes

           Introduction to antibodies - Tech handbook from Chemicon

       Immunohistochemistry

           Immunocytochemistry resources collection

           Troubleshooting tips of IP, western and immunostaining

           Introduction to antibodies - Tech handbook from Chemicon

  Cell biology

       Apoptosis and proliferation

           Quantification of Apoptosis and the Cell Cycle Distribution of Primary B Cells Using PI

           Caspase assay protocol

           DNA Fragmentation Assays for Apoptosis Protocol

           MTT Cell Proliferation Assay

           Guide to Cell Proliferation and Apoptosis Methods

       Cell culture

           ES Cell Culture and Manipulation

           Instruction Manual for Gene Targeting

           Protocol of transgene construct and ES cell prepartion

       Cell cycle analysis

           Flow cytometry protocols

       Cell motility

       Extracellular matrix

           Cell adhesion protocol

       In situ hybridization

           In situ hybridization protocols

           Practical in Situ Hybridization

           In situ hybridization pdf file from GeneDetect

       Nuclear and organelles

       Protein trafficking

       Signal transduction

           Signal Transduction: Glossary and Methods

  Genetics

       Cytogenetics

           Cytogenetics culture and harvest

           Cytogenetics staining protocols from UW Waisman Center

       Footprinting

           DNase I footprinting

           Detection of Alu by PCR-A Human DNA Fingerprinting Lab Protocol

       Genotyping

           Protocol for Genotyping using AP-RDA markers

       Genetic Mapping

           Gene Mapping (Amersham)

  Bioinformatics

       Criticisms and Limitations of Impact Factors

       Tutorial on bioinformatics

           Bioinformatic tutorials

           Lecture Notes on Biological Sequence Analysis

           Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids

           GenBank

           Guide to Selected Bioinformatics Internet Resources

           Parsing Protein Domains with Perl

           Seminar in Bioinformatics from Indiana University

           Online Lectures on Bioinformatics

       Online analysis tools

           Online Tools from Labvelocity

           Promoter database

       Software for biologists

           Free Software in Molecular Biology

       Programming for bioinformatics

           Lecture Notes on Biological Sequence Analysis

           Beginning Perl for Bioinformatics

           Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids

           Bioinformatics software development at bioinformatics.org

           Perl Programming Course for Bioinformatics and Internet

           bioperl - open source Perl tools

           Synergistic use of promoter prediction algorithms

           Journal impact factor of 1999 and 2000

       Online protocol resources

           bioprotocol.com - lab protocols

           BBHelp: Molecular Biology Help

           Links to Yeast Protocols on the Web

           Biovisa - collection of online protocols

           Protocols used at NCI