Title: Intelligent image-based deformation-assisted cell sorting with molecular specificity
Author: Ahmad Ahsan Nawaz, Marta Urbanska, Maik Herbig, Martin Ntzel, Martin Krter, Philipp Rosendahl, Christoph Herold, Nicole Toepfner, Markta Kubnkov, Ruchi Goswami, Shada Abuhattum, Felix Reichel, Paul Mller, Anna Taubenberger, Salvatore Girardo, Angela Jacobi, Jochen Guck
Abstract: Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels.