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共同学习ICAT－蛋白组的方向 [精华]

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biodna

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这个帖子发布于17年零162天前，其中的信息可能已发生改变或有所发展。

先来点快餐
Isotope-Coded Affinity Tags (ICAT) Methodology - Flash Animation
http://www.bio.davidson.edu/Courses/genomics/ICAT/ICAT.html
The Royal Society of Chemistry 的资料
http://www.chemsoc.org/exemplarchem/entries/2002/proteomics/icat.htm
National Center for Toxicogenomics (NCT) 的资料
http://dir.niehs.nih.gov/proteomics/emerg2.htm

最初的文献：
Gygi, SP, Rist, B, Gerber, SA, Turecek, F, Gelb, MH, and Aebersold, R. 1999. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nature Biotechnology, 17:994-999.
http://www.nature.com/cgi-taf/DynaPage.taf?file=/nbt/journal/v17/n10/abs/nbt1099_994.html&dynoptions=doi1064812543
谨此抛砖引玉！

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2003-09-29 13:32 浏览 : 3628 回复 : 17

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biodna 编辑于 2003-09-29 20:05

• 山东一乡镇卫生院院长荣获全国脱贫攻坚先进个人荣誉称号

难得清醒

胃肠外科

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好！谢谢

2003-09-29 14:09

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• (求大佬指点）医学专硕毕业，四证合一，在深圳找得到工作吗

codegreen

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俺看过一些文献，但没有实际操作经验，主任可否谈谈？

2003-09-29 16:55

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• 研究生和编制都考上了选哪个

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biodna

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鄙人也正学呢，从基础开始

Some glossary of experimental terms used in proteomics
2DE
Two-dimensional gel electrophoresis is the separation of proteins using two orthogonal parameters, isoelectric oint (charge) and relative molecular mass, which are both usually determined on the basis of protein mobility in a polyacrylamide gel matrix.
Discovery science
Investigation of a biological system or process by enumerating the elements of a system irrespective of any hypotheses on how the system might function.
ESI
The electrospray ionization process is achieved by spraying a solution (such as the effluent of a HPLC column) through a charged needle at atmospheric pressure towards the inlet of the mass spectrometer; the voltage applied to the needle tip and a pressure differential result in the formation of ions for mass analysis and their transfer into the mass spectrometer.
ICAT
Isotope-coded affinity tag reagent comprising a chemical modifying group linked to an affinity group through a mass-encoded linker.
Ion source
Mass spectrometer component designed to use the principles of an ionization method for generating ions (charged analytes) for mass analysis.
Ionization
Process of adding charge onto an uncharged (neutral) analyte, in other words, the formation of an ion; either ionization is conducted in a vacuum or ions formed at atmospheric pressure are transferred into the vacuum system of the mass spectrometer.
MALDI
Matrix-assisted laser desorption ionization is a process by which ion formation is promoted by short laser pulses; the sample is deposited on a sample plate into the source (which is held under vacuum) and then embedded in a matrix that promotes ionization; a laser fired at the sample that is co-crystallized with the matrix results in the desorption of the analyte from the sample plate and its ionization.
Mass analyzer
Mass spectrometer component that can measure the mass-to-charge ratio of charged molecules (ions); ion-trap, quadrupole and time of flight (TOF) analyzers are used most often.
Mass spectrometry
Accurate mass measurement of charged analytes (ions); in the context of proteomics, analytes are usually peptides or less frequently protein ions; a mass spectrometer measures the mass-to-charge ratio of charged species under vacuum and comprises, broadly, an ionization source and a mass analyzer.
MS spectra
Single-stage mass spectrometry spectra provide mass information on all ionizable components in a sample; these data are used, for example, for peptide mass fingerprinting.
MS/MS spectra
MS/MS spectra are generated in instruments equipped with a mass filter that can select a peptide ion from a mixture of peptide ions, a collision cell in which peptide ions are fragmented into a series of product ions (through collision of the selected precursor ion with a noble gas in a process referred to as CID), and a second mass analyzer that records the fragment ion mass spectrum; the fragment ion spectra are referred to as either MS/MS spectra or CID spectra.
Protein identification
Method to determine the sequence identity of a protein; two common mass spectrometry–based approaches used are peptide mass mapping and searching uninterpreted MS/MS spectra; in both methods, observed data are matched to theoretically derived peptide and/or fragment ion masses calculated from sequence databases.
Systems biology
Study of a biological system by the systematic and quantitative analysis of all of the components that constitute the system.

2003-09-29 20:04

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