别人给的质粒，浓度280ng/ul，转化没长菌？电泳跑不出？酶切没反应？
同批次其他质粒做对照，没有以上问题，哪位大神指导指导啊
不知道邀请谁？试试他们
Do you havew this plasmid DNA map?
If you have map, try to find those enzyme sites and do testing cut with the original plasmid.
