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植物与农林科学

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论坛首页  >  植物学与农林科学讨论版   >  生命科学前沿
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【编译】植物与农林科学讨论版科研最新动态-66

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楼主 donggua
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这个帖子发布于6年零306天前,其中的信息可能已发生改变或有所发展。
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http://www.dxy.cn/bbs/topic/25950307
66 from scientific reports
A complete ancient RNA genome: identification, reconstruction and evolutionary history of archaeological Barley Stripe Mosaic Virus
The origins of many plant diseases appear to be recent and associated with the rise of domestication, the spread of agriculture or recent global movements of crops. Distinguishing between these possibilities is problematic because of the difficulty of determining rates of molecular evolution over short time frames. Heterochronous approaches using recent and historical samples show that plant viruses exhibit highly variable and often rapid rates of molecular evolution. The accuracy of estimated evolution rates and age of origin can be greatly improved with the inclusion of older molecular data from archaeological material. Here we present the first reconstruction of an archaeological RNA genome, which is of Barley Stripe Mosaic Virus (BSMV) isolated from barley grain ~750 years of age. Phylogenetic analysis of BSMV that includes this genome indicates the divergence of BSMV and its closest relative prior to this time, most likely around 2000 years ago. However, exclusion of the archaeological data results in an apparently much more recent origin of the virus that postdates even the archaeological sample. We conclude that this viral lineage originated in the Near East or North Africa, and spread to North America and East Asia with their hosts along historical trade routes.
http://www.nature.com/srep/2014/140206/srep04003/full/srep04003.html
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2014-05-07 15:35 浏览 : 5441 回复 : 2
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认领。。。
2014-05-23 19:40
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A complete ancient RNA genome: identification, reconstruction and evolutionary history of archaeological Barley Stripe Mosaic Virus
一个古老RNA的全基因组:考古学中的大麦条花叶病毒的鉴定,重组和进化史
The origins of many plant diseases appear to be recent and associated with the rise of domestication, the spread of agriculture or recent global movements of crops. Distinguishing between these possibilities is problematic because of the difficulty of determining rates of molecular evolution over short time frames. Heterochronous approaches using recent and historical samples show that plant viruses exhibit highly variable and often rapid rates of molecular evolution. The accuracy of estimated evolution rates and age of origin can be greatly improved with the inclusion of older molecular data from archaeological material. Here we present the first reconstruction of an archaeological RNA genome, which is of Barley Stripe Mosaic Virus (BSMV) isolated from barley grain ~750 years of age. Phylogenetic analysis of BSMV that includes this genome indicates the divergence of BSMV and its closest relative prior to this time, most likely around 2000 years ago. However, exclusion of the archaeological data results in an apparently much more recent origin of the virus that postdates even the archaeological sample. We conclude that this viral lineage originated in the Near East or North Africa, and spread to North America and East Asia with their hosts along historical trade routes.
大多数植物病害大多近期才发现,并与植物驯化,农业发展或作物的全球移动相关。区分这些可能性比较困难因为在短时间内很难决定分子进化速率。通过近期和过去的样品发现植物病毒展示高度多样性和进化速率。可以通过加入历史样品中获得的较老的分子数据来极大促进进化速率的估计和起始岁月的准确性。
本文首次重新组装了一个古老的大麦条纹病毒的基因组序列,该病毒从750年前的大麦上分离得到。包含该分离物的系统树分析发现大麦条纹花叶病毒和它最亲近的分离物的差异先于这个时间。很可能在2000年之前。但不包含这个古老数据的话,得到的结论是病毒源自出现样品之后。
病毒谱系源自近东或北非,伴随着他们的寄主通过历史上的贸易路线传播到北美和东亚。
================================
个人理解,在分析病毒进化过程中,采用的数据十分重要,尤其是包含一些比较古老的分子数据(比如从文物中得到的样品),这样会极大的提高准确性。
2014-05-23 19:40
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