各位大神请教一下,我最近用southern blot想确定质粒在细胞内的整合方式,用了两种酶切,一种酶切只有一条带,另一种酶切又有四条带,请问这个结果怎么解释,是有四个整合位点吗?
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Please label the blot picture. Please describe the probe DNA and show the restriction enzyme mapping.
详细情况我已列出来了,麻烦大神指导下
Are sample DNA genomic or plasmid? On the blot which lane is the sample DNA cut with BamH I, and which with Nhe I?