小闯ing

麦克林托克

请问这个软件就可以对免疫荧光的图片进行荧光定量吗？我是在文献中看到的

Endothelial monolayers were imaged using an Olympus IX81 (Olympus America, Center Valley, PA, USA) fluorescence microscope and analyzed using MetaMorph software (Molecular Devices, Sunnyvale, CA, USA). ZO-1 tight junction immunostaining was quantified by applying an identical threshold for ZO-1 immunofluorescence to five randomly selected images of each endothelial culture. Consistent with methods previously described,^{7, 8} ZO-1 immunostaining was quantified as the area percentage of an image with fluorescence above a predetermined threshold, normalized to the total number of cells in each image. Immunostaining images were randomly selected from each experimental condition, and an identical quantitative analysis method was applied to all samples.