最近在做northern blot,但总杂到核糖体rna,该怎么解决这问题
不知道邀请谁?试试他们
Washing blots with more stringent condition and increasing the temperature during washing.
mparkWashing blots with more stringent condition and increasing the temperature during washing.
0.1*SSC,68度洗两次,每次15min。换了3种不同的探针了,也是一样的结果。并且实验室有做northern的同学,也都杂到rRNA,实验室老师说以前从没遇过,突然就出现了。
Can you detail the hybridization buffer?