最近在做northern blot，但总杂到核糖体rna，该怎么解决这问题
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Washing blots with more stringent condition and increasing the temperature during washing.
mparkWashing blots with more stringent condition and increasing the temperature during washing.
0.1*SSC，68度洗两次，每次15min。换了3种不同的探针了，也是一样的结果。并且实验室有做northern的同学，也都杂到rRNA，实验室老师说以前从没遇过，突然就出现了。
Can you detail the hybridization buffer?
