jiandong0016?
张老师，您好
我现在在国内一所高校读博，方向是微生物与生物物理，打算两年后出去，想问一下现在需要准备些什么，三四点的文章一两篇有说服力吗？如果想去有名的研究机构，会不会有文章的申请门槛？

张科宏老师
征得作者同意，下面对 John Smith 申请博士后的 cv 和 cover letter（所有姓名均为化名；论文保留发表杂志和年份，省略期、卷）进行公开讨论，希望大家提供指出文件中缺点、错误，并提出修改建议（越详细越好）， 同时也相信同学们能在讨论中有所收获。

CV：

EDUCATION AND QUALIFICATION

2002, 4 - 2006, 3 Ph.D., Advised by Prof. A. Sakura, Nagoya University School of Medicine, Japan Project Title: Roles of lipid rafts and ganglioside GM1 in metastasis of Lewis lung cancinoma
? Established high-metastatic sublines by repeatedly injecting Lewis lung carcinoma (P29) into C57BL/6 mice with i.v. or s.c. injection
? Found that integrins seemed to be enriched in the GEM/rafts by reduced GM1 levels, and subsequently MMP-9 was recruited to the GEM/rafts, resulting in its efficient secretion and activation, and eventually in the increased invasion and metastatic potentials in the high-metastatic cells.

1996, 4 - 1999, 3 Master of Agriculture, Advised by Prof. B. Sakura, Gifu University School of Agriculture, Japan
Project Title: Effects of casein or ovomucin on cholesterol metabolism in rats and Caco-2 cells
? Designed a lipid metabolism model in rats and a cholesterol absorption model in Caco-2 cells
? Found that egg ovomucin could attenuate hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells.

1988, 9 - 1993, 7 Medical Doctor, TianJin Medical University, china

EXPERIENCE

2006, 4 - Present 　 Research Fellow Department of Biochemistry II Nagoya University School of Medicine, Japan
Project Title: Identify molecules and elucidate the mechanisms involved in metastasis of Lewis lung cancinoma
? Trying to identify the novel metastasis-associated genes using gene-chip approach with high-metastatic sublines and parent lines.
? Analyze the regulation of ganglioside GM1 on VEGF-A expression in lymphatic metastasis of Lewis lung cancinoma.
? Analyze the interaction of integrins and Rho activity associated molecules in metastasis of Lewis lung cancinoma.
? Supervise the research programs of undergraduate and postgraduate students.

SKILL & ABILITIES
? Genetic engineering: DNA and RNA extraction, Library manufacture, plasmid transfection, northern and western blotting, RT-PCR and real-time RT-PCR, establishment of cell lines, etc.
? Animal experiment: i.v. and s.c. injection, establishment of animal model.
? Protein engineering: extraction and the refinement of protein, chromatography, coimmunoprecipitation, ELISA, DNA microarray, immunofluorescence, etc.
? The others: flow cytometric analysis, gelatin zymography, MTT assay, matrigel invasion assay, extraction and analysis of gangliosides, etc.

Research interest
? Mechanisms in metastasis of cancer
? Genetic therapy of cancer

Awards and Honors
? 1997 – 1999, Honors Scholarship for Privately Financed International Students (Gifu University School of Agriculture)
? 2003 – 2006, Japanese Government MEXT Scholarship Award (Nagoya University School of Medicine)

GRAND
? 2007, Grants-in-Aid for Scientific Research, Young Researcher B, Japan, $30,000 For a research project: the interaction of integrins and Rho activity associated molecules in metastasis of Lewis lung cancinoma
? 2006, The Open Clusters Program of Nature COE, $5,000 For a research project: Identify molecules and elucidate the mechanisms involved in metastasis of Lewis lung cancinoma

MAIN PUBLICATIONS
1. --- Genetic mechanisms for the synthesis of fucosyl GM1 in small cell lung cancer cell lines. Glycobiology. 2006 ---
2. --- Down-regulation of caveolin-1 in mouse Lewis lung cancer P29 is a causal factor for the malignant properties in a high-metastatic subline. Oncol Rep. 2006 ---
3. --- Metastatic Potential of Mouse Lewis Lung Cancer Cells Is Regulated via Ganglioside GM1 by Modulating the Matrix Metalloprotease-9 Localization in Lipid Rafts. J Biol Chem. 2006 ---.
4. --- Mechanisms for the apoptosis of small cell lung cancer cells induced by anti-GD2 monoclonal antibodies: roles of anoikis. J Biol Chem. 2005 ---.
5. --- Egg ovomucin attenuates hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells. Lipids 2002 ---.

Cover Letter：

Dear Dr. ,

Would you forgive me to suddenly send this E-mail to you?
I am now a postdoctoral fellow in the Department of Biochemistry at Nagoya University Graduate School of Medicine, from which I graduated in Apr. of 2006. In the department, I studied on the mechanisms of cancer metastasis using a mouse Lewis lung cancer with focus on the roles of ganglioside GM1 under supervision of Professor A. Sakura. In this study, I could demonstrate the role of GM1 to suppress cancer metastasis by dispersing integrins from lipid rafts and consequently suppress the secretion of MMP-9. This study could be published in the Journal of Biological Chemistry (Smith. et al: J. Biol. Chem. ---, 2006). Then, I have studied on the identification of essential molecules in cancer metastasis by using DNA array, and have already found very interesting molecules that show marked up-regulation in the high metastatic sublines of Lewis lung cancer.
Now, I hope to study more about the mechanisms of cancer metastasis in the United States. Furthermore, I would also learn about the development of new cancer therapy utilizing these findings for the application in clinical fields. If possible, I would like to work in your laboratory as a postdoctoral fellow. As shown in my bibliography, I have learned variety of technologies on cell biology, biochemistry and immunology. I am also very eager to learn new techniques needed in the new projects. So, I would very much appreciate if you would consider about me to accept as a postdoctoral fellow.
Please find my resume enclosed. It will provide you with details relevant to my experience and education. The attachment is in Acrobat PDF format, so if you have any problem in opening it, please let me know and I can send you a hard copy.
I am looking forward to hearing from you soon.

Sincerely yours,

John Smith