该话题已被锁定 - 丁香园 , 2013-10-23 18:04
如果您尚不清楚该话题被锁定的原因,请参考论坛规则以及本版公告或者联系本版版主

【交流】病毒包装答疑,你有哪些不解之处?

thphoon
常驻站友

医师认证骨科(医学生)

2013-05-31 10:17 消息 引用 分享
hanbio wrote:
您好!包装出来的病毒滴度低,您可以浓缩后再进行目的细胞感染哦,一般可以反复感染两三次的,中间间隔要12h以上O(∩_∩)O~
谢谢你的回答
hanbio
常驻站友

2013-05-31 10:34 消息 引用 分享
kiki-immu wrote:
请问hanbio几个问题:

1. 纯化后的逆转录病毒颗粒如何保存?保存液是什么?

2、在收集培养上清不立刻做纯化(想攒多些培养上清再一起纯化),这些培养上清又如保存?

3、我做的逆转录病毒包装,请问目的蛋白在包装细胞或者病毒滴定细胞中表达吗?

先谢了!

@hanbio
您好!纯化后的病毒用无血清的DMEM保存就可以了,一般放在-80度进行长久保存;如果收集的上清不想立即纯化可先放在-80度,等下次收集的一起纯化;目的蛋白在包装细胞中或者滴度测定细胞中会表达的,滴度测定就是根据目的蛋白表达情况来进行操作的O(∩_∩)O~
hanbio
常驻站友

2013-05-31 10:45 消息 引用 分享
shihaishui wrote:
倒不是稳定表达,我的实验周期比较长,所以选择了慢病毒。我现在的病毒滴度是E8,我要做脑区注射,所以单次注射体积最多2ul,如果是只未注射一次慢病毒,我怕最后的感染率低,所以我想把包装好的慢病毒(大概400ul)多次脑区注射(2ul/次/12h 计划未注射3-4次),这样进入同一脑区的病毒总数就多了,理论上侵染率也会提高
不知道这种方法可行不可行

谢谢您对回复!
您好!这样可以的,每次注射的病毒体积是不能大的,否则对动物会造成伤害,不过这里要和您说一下哦,慢病毒的感染效率是相对比较低的,您那边要做好心理准备哦O(∩_∩)O~
kiki-immu
常驻站友

2013-05-31 12:47 消息 引用 分享
hanbio wrote:
您好!纯化后的病毒用无血清的DMEM保存就可以了,一般放在-80度进行长久保存;如果收集的上清不想立即纯化可先放在-80度,等下次收集的一起纯化;目的蛋白在包装细胞中或者滴度测定细胞中会表达的,滴度测定就是根据目的蛋白表达情况来进行操作的O(∩_∩)O~
谢谢hanbio,我不明白再来问您,后面一句话不太理解,我先看看相关内容!
kiki-immu
常驻站友

2013-05-31 12:51 消息 引用 分享
继续咨询两个问题:

1、加入4倍体积的2.5M Nacl配制的20%的(w/v)的PEG-8000,混匀后4度震荡过夜,然后12000g 4度 30分钟,然后收集沉淀即可。

上述方法可以达到纯化病毒颗粒的效果吗?

2、收集病毒上清,去除细胞碎片,离心机一般采用多大的转速?

先谢了!
thphoon
常驻站友

医师认证骨科(医学生)

2013-05-31 15:26 消息 引用 分享
相关疾病:
有一个低级的问题想请教一下,就是我们用病毒感染细胞的时候,对细胞的融合度有一个要求,一般是50%-80%,我有点不明白,为什么要这个融合度呢,细胞融合度会影响病毒感染效率吗?
hanbio
常驻站友

2013-06-03 09:51 消息 引用 分享
kiki-immu wrote:
继续咨询两个问题:

1、加入4倍体积的2.5M Nacl配制的20%的(w/v)的PEG-8000,混匀后4度震荡过夜,然后12000g 4度 30分钟,然后收集沉淀即可。

上述方法可以达到纯化病毒颗粒的效果吗?

2、收集病毒上清,去除细胞碎片,离心机一般采用多大的转速?

先谢了!
您好!用您说的方法是可以纯化病毒颗粒的哦,收集病毒上清,一把2000g就可以去掉细胞碎片了O(∩_∩)O~
hanbio
常驻站友

2013-06-03 09:52 消息 引用 分享
thphoon wrote:
有一个低级的问题想请教一下,就是我们用病毒感染细胞的时候,对细胞的融合度有一个要求,一般是50%-80%,我有点不明白,为什么要这个融合度呢,细胞融合度会影响病毒感染效率吗?
您好!细胞长的太满或者太稀时细胞活性都不太好,细胞活性不太好时,可能就会影响病毒的感染效率的O(∩_∩)O~
hanbio
常驻站友

2013-06-03 09:53 消息 引用 分享
zfb426
铁杆站友

2013-06-03 10:49 消息 引用 分享
你好,请问包装好的慢病毒颗粒去感染细胞,在细胞房的超净台操作有没有危险?我们实验室没有病毒房,有什么好点的方法弥补?
kiki-immu
常驻站友

2013-06-03 14:48 消息 引用 分享
hanbio wrote:
您好!用您说的方法是可以纯化病毒颗粒的哦,收集病毒上清,一把2000g就可以去掉细胞碎片了O(∩_∩)O~

Thank you very much!
hanbio
常驻站友

2013-06-04 09:56 消息 引用 分享
zfb426 wrote:
你好,请问包装好的慢病毒颗粒去感染细胞,在细胞房的超净台操作有没有危险?我们实验室没有病毒房,有什么好点的方法弥补?
那就在超净台里操作好了,操作的时候超净台不能开风,要带好口罩,手套,穿好白大褂,用完的器材要妥当处理O(∩_∩)O~
andybcf
入门站友

2013-06-05 15:47 消息 引用 分享
您好!我想请问一下用293T细胞包装慢病毒时,根据您的经验是氯化钙法好还是阳离子法转染好?或有其他的更好的转染方法吗?
陈勇一无所有
常驻站友

2013-06-05 17:33 消息 引用 分享
随着分子生物学的理论及技术方法(特别是重组DNA技术)的迅速发展,人们可以在实验室构建各种载体、克隆及分析目标基因,使疾病深入至分子水平研究,于是诞生了基因诊断、基因治疗技术。

基因治疗从基因角度理解是对缺陷的基因进行修复增补,或将正常有功能的基因置换的方法。 从治疗角度理解是一种基于导入遗传物质以改变患者细胞的基因表达从而达到治疗或预防疾病的目标的新措施。

当代基因治疗研究的热门方法是将外源基因DNA或RNA片段导入靶细胞或组织,研究靶基因的上调或抑制情况。例如,有些异常基因(癌基因、病毒基因),可通过反义核酸技术引入外源片段将其抑制;有些基因本身有治疗作用,体外合成该基因导入体内使其表达丰度提高。

故选择合适高效的基因导入介质系统尤为关键。而病毒载体已成为当前基因治疗载体研究的热点,其优势在于:

1、病毒包装技术经历了多年的研究,已趋于成熟,可用于产业化大量生产。

2、病毒基因组的结构简单、分子背景比较清楚,稳定易于改造、易于制备。

3、病毒的宿主范围广,且具有高效的靶向特异性。

4、在目的基因表达方面相对于脂质体介导的效果更明显、长时、稳定。

5、通过载体改造的方式形成了复制缺陷型结构,安全性高。
grehxp
铁杆站友

医师认证传染科

2013-06-06 16:21 消息 引用 分享
想以慢病毒为载体做抗菌肽的真核分泌表达,查了很多资料,似乎真核表达很难做,是这样吗?
hanbio
常驻站友

2013-06-06 22:35 消息 引用 分享
andybcf wrote:
您好!我想请问一下用293T细胞包装慢病毒时,根据您的经验是氯化钙法好还是阳离子法转染好?或有其他的更好的转染方法吗?

氯化钙法转染效率效率相对低,出毒效率相对低。且对氯化钙法对pH值要求很苛刻,差一点都不行,不建议用。
阳离子脂质体方法比较好,但常规使用的Lipo2000细胞毒性大,有钱的可以使用低毒性的Fugene HD,钱少的可以选用我公司的Lipofiter阳离子脂质体产品,效率和Lipo2000差不多,毒性基本没有。我们自己包病毒就用Lipofiter。
hanbio
常驻站友

2013-06-06 22:49 消息 引用 分享
grehxp wrote:
想以慢病毒为载体做抗菌肽的真核分泌表达,查了很多资料,似乎真核表达很难做,是这样吗?
抗菌肽比较短,需要先分析是否具备分泌的前导肽,如果没有,构建的时候在N端加前导肽应该可以分泌表达。具体需要再进行reference的查找阅读。
蜡笔哈哈
入门站友

2013-06-09 10:42 消息 引用 分享
相关疾病:
老师你好,我一直在做慢病毒包装,但是测滴度都只有10E3到10E4,我是用的钙转法,参照的是nature protocol。请问用脂质体能达到多少滴度?我感染的是HepG2细胞,大概要多大的滴度能比较好的感染?
hanbio
常驻站友

2013-06-10 12:03 消息 引用 分享
蜡笔哈哈 wrote:
老师你好,我一直在做慢病毒包装,但是测滴度都只有10E3到10E4,我是用的钙转法,参照的是nature protocol。请问用脂质体能达到多少滴度?我感染的是HepG2细胞,大概要多大的滴度能比较好的感染?


这个滴度基本属于不能用的鸡肋病毒,建议如下:
1.你的病毒如果带荧光,可通过观察确定包毒转染时的转染效率,低于70%的话后面都不用做了。
2.包病毒需要良好的细胞状态,较高的转染效率(包括优质的质粒和高效的转染试剂),合适的质粒配比体系,以上缺一不可,你的问题不一定是某一方面的问题,但首先建议先换钙转为脂质体转染,逐一排除可能原因。我们不建议用钙转,不稳定,特别新手更容易出问题。如果你有需要,可向我们申请脂质体转染试剂试用装进行同步操作对比。
3. HepG2 慢病毒MOI大概需要30,可以用polybrene助转。
ps 病毒包装的条件优化与摸索是个长期且工作量巨大的工程,请做好长期抗战准备。如果需要我们的支持,可点击此链接与我们QQ交流:http://wpa.b.qq.com/cgi/wpa.php?ln=1&key=XzgwMDA1Mjg2M18zMjQwMF84MDAwNTI4NjNfMl8
学习勤快
铁杆站友

2013-06-11 08:32 消息 引用 分享
见邀请函。一直未会函。出于礼节,我问一个问题。08年我只是主要研究20面体外壳的“无棱病毒”。结构原则。
我就此提问:病毒包装工程师王老师,请举一实例讲解。注意是20面体的。
其他各位先生亦可以给我讲解。致谢。
基因组学我感兴趣!
学习勤快 edited on 2013-06-11 08:35 举报
木木小世界
入门站友

2013-06-11 23:00 消息 引用 分享
相关疾病:
老师,我最近开始做慢病毒,操作一直是没问题的,我将24h收集的病毒上清再去感染293T细胞,并设了梯度。发现只要200ul转染效率就有80-90%,然而我用了1ml去感染目的细胞,视野里却没几个。不知道问题出在哪里?病毒包转有没有成功呢?
hanbio
常驻站友

2013-06-13 10:22 消息 引用 分享
相关疾病:
木木小世界 wrote:
老师,我最近开始做慢病毒,操作一直是没问题的,我将24h收集的病毒上清再去感染293T细胞,并设了梯度。发现只要200ul转染效率就有80-90%,然而我用了1ml去感染目的细胞,视野里却没几个。不知道问题出在哪里?病毒包转有没有成功呢?
您好!病毒量加的多了对细胞是有毒性的哦,您可能就是1ml的病毒太多了,导致细胞死亡了或者细胞活性不好了,从而使得感染效率降低了,既然您200ul感染的效率就很高了,你就没必要加大病毒量了哦O(∩_∩)O~
gwh123
入门站友

2013-06-13 11:46 消息 引用 分享
相关疾病:
您好,我最近在做某个基因启动子的慢病毒包装,并将慢病毒感染到肿瘤细胞中,收集稳定表达的细胞。包装质粒由公司合成,是PLVX系统,该质粒抗嘌呤霉素,不管是公司提供的慢病毒还是自己包装的慢病毒,感染细胞48h后,用含5ug/ml嘌呤霉素的培养基筛选细胞,感染的细胞中一部分细胞死亡,未感染的对照组细胞加嘌呤霉素后则完全死亡,但是在荧光显微镜下,这些经过嘌呤霉素筛选的细胞也不发绿色荧光,重复好几次都是这种情况,实在是没辙了,请老师帮忙解答,不知是否是系统本身有问题还是其他的情况。
[img]data:image/png;base64,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****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
gwh123
入门站友

2013-06-13 11:48 消息 引用 分享
这是293T细胞包装慢病毒的图片,但是感染细胞就不亮
蜡笔哈哈
入门站友

2013-06-13 12:56 消息 引用 分享
相关疾病:
hanbio wrote:
这个滴度基本属于不能用的鸡肋病毒,建议如下:
1.你的病毒如果带荧光,可通过观察确定包毒转染时的转染效率,低于70%的话后面都不用做了。
2.包病毒需要良好的细胞状态,较高的转染效率(包括优质的质粒和高效的转染试剂),合适的质粒配比体系,以上缺一不可,你的问题不一定是某一方面的问题,但首先建议先换钙转为脂质体转染,逐一排除可能原因。我们不建议用钙转,不稳定,特别新手更容易出问题。如果你有需要,可向我们申请脂质体转染试剂试用装进行同步操作对比。
3. HepG2 慢病毒MOI大概需要30,可以用polybrene助转。
ps 病毒包装的条件优化与摸索是个长期且工作量巨大的工程,请做好长期抗战准备。如果需要我们的支持,可点击此链接与我们QQ交流:http://wpa.b.qq.com/cgi/wpa.php?ln=1&key=XzgwMDA1Mjg2M18zMjQwMF84MDAwNTI4NjNfMl8
我钙转的方法是:
第一天:60mm板铺293T细胞

第二天:观察细胞70%左右转染。

转染步骤:
1.向293T培养液中加入3ul 25mM氯喹,至终浓度为25uM
2.准备1.5ml无菌tube,加上各个质粒pCDH-eGFP 10ug;pCMV-VSVG 10ug;p8.2 10ug,用ddH2O定容至150ul
3.加37.2ul 2M CaCl2,吹打20下,能看到絮状物出现
3.逐滴加入150ul 2X HBS并蜗旋
5.将mix滴加到293T培养皿中,加时不断晃动培养皿
7.6h后换新鲜培养液
转染48h 72h收集病毒,方法:
收集培养液,500g,10min;0.45um PES滤膜过滤(我用的是国产的滤膜)分装至4ml管,4℃保存,在1~2天中即用于感染

转染两天之后观察细胞荧光,在90%以上。但是测病毒滴度不高。
我也用过罗氏的X-tremeGENE做对照,但是转染效果不如钙转。
请问细胞状态怎样算好?293T是不是转染过程中都要状态很好?
蜡笔哈哈
入门站友

2013-06-13 16:16 消息 引用 分享
请问转染60mm皿293T,您用的3个质粒的量分别为多少
lb88911
入门站友

2013-06-18 17:31 消息 引用 分享
老师您好,我之前用oligo做了silence效果不错,现在想包装慢病毒进行后续试验,我想问的问题是,为了保证silence效率,可以用oligo的序列直接包装慢病毒吗?还是要重新设计序列呢?谢谢。
hanbio
常驻站友

2013-06-19 09:41 消息 引用 分享
蜡笔哈哈 wrote:
请问转染60mm皿293T,您用的3个质粒的量分别为多少
您好!绝对比例是1:1:1哦,,总共的质粒量是根据转染试剂操作说明书来决定的O(∩_∩)O~
hanbio
常驻站友

2013-06-19 09:54 消息 引用 分享
lb88911 wrote:
老师您好,我之前用oligo做了silence效果不错,现在想包装慢病毒进行后续试验,我想问的问题是,为了保证silence效率,可以用oligo的序列直接包装慢病毒吗?还是要重新设计序列呢?谢谢。
您好,您可以用您的oligo序列直接包毒的,不过在包毒之前,做好构建到普通质粒上看看效果是否和oligo一样O(∩_∩)O~

ps 也可以构建到病毒质粒上,先用病毒质粒直接转染试试silence efficiency。
hanbio edited on 2013-06-21 00:43 举报
hanbio
常驻站友

2013-06-20 23:11 消息 引用 分享
gwh123 wrote:
您好,我最近在做某个基因启动子的慢病毒包装,并将慢病毒感染到肿瘤细胞中,收集稳定表达的细胞。包装质粒由公司合成,是PLVX系统,该质粒抗嘌呤霉素,不管是公司提供的慢病毒还是自己包装的慢病毒,感染细胞48h后,用含5ug/ml嘌呤霉素的培养基筛选细胞,感染的细胞中一部分细胞死亡,未感染的对照组细胞加嘌呤霉素后则完全死亡,但是在荧光显微镜下,这些经过嘌呤霉素筛选的细胞也不发绿色荧光,重复好几次都是这种情况,实在是没辙了,请老师帮忙解答,不知是否是系统本身有问题还是其他的情况。
你这是要刷屏啊~~~!请提供你质粒的图谱!没有图谱无法分析!

如果需要在线咨询,请点击下方汉恒生物官方QQ链接与我们联系!
http://wpa.b.qq.com/cgi/wpa.php?ln=1&key=XzgwMDA1Mjg2M18zMjQwMF84MDAwNTI4NjNfMl8
木木小世界
入门站友

2013-06-24 14:23 消息 引用 分享
hanbio wrote:
您好!绝对比例是1:1:1哦,,总共的质粒量是根据转染试剂操作说明书来决定的O(∩_∩)O~
老师,我想问问,如果是10cm的皿呢?比例也是1:1:1吗?我做的病毒包装,是用的2:1:2,pMD2G是1,也可以做得出来。这个比例真的有绝对一说吗?
hanbio
常驻站友

2013-06-24 15:33 消息 引用 分享
木木小世界 wrote:
老师,我想问问,如果是10cm的皿呢?比例也是1:1:1吗?我做的病毒包装,是用的2:1:2,pMD2G是1,也可以做得出来。这个比例真的有绝对一说吗?
您好!绝对比例都是1:1:1哦,根据分子量和质粒浓度来算的话,加入的质粒体积肯定是不同的,但是综合分子量来说,绝对比例都是1:1:1O(∩_∩)O~
huangjia10
入门站友

医师认证科室保密

2013-06-24 21:06 消息 引用 分享
想咨询一些关于逆转录病毒包装的问题,我近几个月一直在用psuper-retro-neo-gfp 载体进行逆转录病毒包装,选用的包装细胞有293T,293ft,PlatE,转染后能见98%的荧光,可是48-72小时病毒收集后感染小鼠成骨细胞,却看不到任何荧光。一直找不到哪个环节导致不产毒。转染293T,293FT都加了对应的辅助质粒。
hanbio
常驻站友

2013-06-29 22:32 消息 引用 分享
相关疾病:
huangjia10 wrote:
想咨询一些关于逆转录病毒包装的问题,我近几个月一直在用psuper-retro-neo-gfp 载体进行逆转录病毒包装,选用的包装细胞有293T,293ft,PlatE,转染后能见98%的荧光,可是48-72小时病毒收集后感染小鼠成骨细胞,却看不到任何荧光。一直找不到哪个环节导致不产毒。转染293T,293FT都加了对应的辅助质粒。
这里面可能有:1.没有出毒;2.出毒了但感染不成功
建议如下:先感染293细胞,如果这个都不成功,就可以肯定没出毒,如果成功,则证明是感染不成功。
ps. Retrovirus 只感染分裂旺盛的细胞,你说的小鼠成骨细胞是MC-3T3-E1还是原代?
hanbio
常驻站友

2013-06-29 22:41 消息 引用 分享
如果需要在线咨询,请点击下方汉恒生物官方QQ链接与我们联系!
http://wpa.b.qq.com/cgi/wpa.php?ln=1&key=XzgwMDA1Mjg2M18zMjQwMF84MDAwNTI4NjNfMl8